FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2546.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2546.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434527
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23820.1660477634788331No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC6500.041.26153145
GCCGACC6950.037.546947145
TCTGACC10800.030.202698145
GTATAGG4300.025.2947981
TAGGACA8700.022.498944
CTAGGAC7150.022.307423
GTATAAG8850.022.122231
GTGTATA9350.021.714751
CCCTTAG1700.003090534121.3269861
CCTAGGC1700.003093661321.3232693
CTGCCGC14800.021.060259145
GTCCTAG7450.020.4395561
CCCCTAG14750.019.17261
GGACACA10250.019.095336
CTCTAGG9200.018.916111
GTCCTGA5407.4578566E-1118.7993431
CGATACA10150.018.567963145
AGGACAC11450.018.36035
GTATATG8350.018.236491
CCCTAGT14800.018.125412
Produced by FastQC (version 0.11.2)