FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2545.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2545.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519364
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33480.22035535921609306No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC5500.044.80384145
GCTGCCC5700.031.788069145
CTGCCGC18500.029.77425145
CCTGTAC2752.1464075E-1028.9935848
CTTAAGG2259.125106E-725.773773
GTATAAG8150.024.9147641
GCCGTAA3753.092282E-1023.192574145
ACTGTGC5550.022.2022938
TATACCG3655.7061698E-921.843765
GTCCTAG8700.021.6725711
GTATTAG5101.8189894E-1221.329341
GCTATAC3406.44759E-821.3202133
TACCCCG2053.6739538E-421.2141175
CTAGGAC8100.020.5832183
TAGGACA9350.020.1559664
TATAGCG2256.881691E-419.3284195
CCCTAAG1900.0058819.0841481
CTATACT6500.018.9573424
TATACTG10400.018.8173315
ATAGGAC3953.1304262E-718.3515763
Produced by FastQC (version 0.11.2)