FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2543.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2543.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165921
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55130.2545337526160926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC7800.033.455265145
GCCGACC6100.026.142641145
CTGCCGC22100.025.58344145
GTATAAG11600.024.3771861
AGGCCGT4006.9303496E-1021.7454226
GTCCTAC2404.3628857E-521.147731
GTGTATA15600.019.520981
CCCCTAG23200.019.3767381
GGCCGTA3751.815497E-719.3283717
GCTCGGA7000.018.644531
GTATAAA19850.018.2636031
TATACTG14650.017.8123595
GCCTAAG37050.017.6129151
CCGTATA7850.017.544483145
TCTGACC15850.017.378447145
CCCTAGT24250.017.3385582
TAGGTCG3352.8653205E-517.3101885
CCTAAGC34350.017.3054872
TAAGCCT39200.017.1970334
AAGGCCG4659.18626E-817.1473225
Produced by FastQC (version 0.11.2)