FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2541.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2541.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757648
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42300.24066252173358943No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC20050.11407289741745788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC6500.045.718063145
GCCGACC7400.042.116673145
GTGTATA12550.035.240281
CTGCCGC21200.029.402206145
GTATAAG12250.028.9985433
CTGTACG10700.028.4508469
TGTATAA14900.026.2738482
CCTAGTG15250.026.1462273
CCCCTAG16300.025.7984521
CCCTAGT16550.023.6544022
TCTGACC13150.022.598282145
TATAAGA18550.021.885074
TAACGTT10600.021.8850695
ACGCGTT5350.021.676218145
ATAACGT12200.019.6091124
GTTAAGG11150.019.5074121
CTAGTGA23150.019.4153184
AGTGACC24000.019.3312636
TAGTGAC22650.019.2037875
GAGACAG19150.018.9247829
Produced by FastQC (version 0.11.2)