FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2540.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2540.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086345
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47670.22848570107053245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC9350.027.905632145
GCCGACC7050.027.757198145
CTGCCGC28350.027.354687145
GTATAAG10350.025.919021
GTGTATA13950.019.7499771
TATACCG7000.019.6761385
TATAAGA19350.019.1066232
CTAGGAC4806.684786E-918.1231923
TAGGACA6603.6379788E-1217.5740054
CTTAGGC31450.017.2876243
GGCTTAG31300.017.141331
CTGTACC9750.017.1008594
CATCTGC23850.017.017693145
GCTTAGG30400.016.9308782
TATCCCG5602.4610927E-916.8282745
CCCTGTG10400.016.7282989
TTAGGCT34250.016.720964
CTATACT8350.016.4953614
TAATAGT13200.016.475634
CTATTTG61050.016.3889311
Produced by FastQC (version 0.11.2)