FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2539.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2539.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072030
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22600.21081499584899677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC3900.050.172173145
GTATAGG2853.237801E-1027.9842831
CTGCCGC11400.027.33552145
CCCCTAG9800.023.6750161
GTGTATA7050.023.6540261
GTCCTAG5400.022.8255671
GTATTAG3854.274625E-1022.5988791
ACCGTGC2252.65713E-522.5496628
CCTAGTG9800.021.4504783
ACTGTGC3406.450318E-821.3179598
CCCTAGT10300.021.1139452
GTATAAG5201.8189894E-1220.914831
GTATAGA5550.020.9022661
ATAGCCG2455.124744E-520.7088748
CCTAGAG6050.020.3684793
AGCCCGT1800.0043137920.1336278
TCCTAGA7850.019.3925512
CACCGTG3051.2784743E-519.0105387
ACAGTGC3852.3919893E-718.826258
TACCGTG2701.0810394E-418.7922635
Produced by FastQC (version 0.11.2)