FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2537.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2537.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955656
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16960.17746971713671028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC4150.038.416897145
GTCCTAG4350.026.6689411
CTGCCGC11400.025.427454145
TACTAGA3753.074092E-1023.2007642
GCCGACC4205.2750693E-1122.430647145
GGGTATG3604.84215E-922.1546651
GTATAAG5251.8189894E-1220.7138823
GTTAGGT2505.9750386E-520.2996033
GTATAGG3651.3587669E-719.8647061
TTATACT7950.019.150574
CATCTGC10700.018.963652145
CCCTAGT9200.018.9136662
CTAGGAC4603.932655E-918.9126763
GTATAGC1950.006841107718.5893823
GTACAGG3151.6821648E-518.4142671
TATACTT7550.018.2446785
TAGCACT2400.001059801718.1246474
CTACTAG2050.00912163717.6844351
CCTAGAG5751.8371793E-1017.651833
AATACCG5451.70985E-917.293245
Produced by FastQC (version 0.11.2)