FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg25362.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg25362.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.509915014164306No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG80.4532577903682719No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC50.28328611898017No Hit
CCCATACCCCACTTACAAGAGATGGAAAGGAACTGTAGGAAGTTCTACAC30.16997167138810199No Hit
ATGATGATGACATTCGAAACCATCTCCGGTTTCTGCTTTTGGTTTTTGAG20.11331444759206798No Hit
ATATAACGCTTCATTTTCGTTTTTATTCTATTTTTTTTCAAATCGGATTT20.11331444759206798No Hit
AGCGGAGGGAGGGGGAAGAGAGGATTTAATACAGTACTTTTTTTAGAGGA20.11331444759206798No Hit
GATCCAGGTTGTCCAAGATCTTGTCCACTGGCACGACCATTTCCTTGAAC20.11331444759206798No Hit
GATTACCCATATTCTAGGCCAGTCGCCTGCATACTCGTCTCTACATAATG20.11331444759206798No Hit
CATTTACTCTACCCTGATCTTTTACCTTCAACTATTTTGTAGTCTCCTTG20.11331444759206798No Hit
GGCTAACCAACGGATTATCAATGGAGCGTGTTTGGTCACAGGGATGCAGA20.11331444759206798No Hit
GTCCGATGCGTTTCGGTCGAAAAGCTCGAATGCTGTCAAATAAAGTGCGT20.11331444759206798No Hit
ACTCCGCACTCTCAACCGCCTGCTGGACTCAAAAAAGGTATTTCCGTTTT20.11331444759206798No Hit
CGGAGGAAGAGTGATATTCTTTTGAAATCCATTCCATCCATCATAATACT20.11331444759206798No Hit
AATAGAAATGAGTCCTTTGATTCTTTTTTTGTGTTTTTGAAGTCGCGACC20.11331444759206798No Hit
CAAATGGAGATTATGTGTGTTCAGTTCCACAAAATAAATGAATCAAATCA20.11331444759206798No Hit
AACATGTTCAGAAATAGATTAAAACTGAAAAACTTACAAAAAATCATTCC20.11331444759206798No Hit
CTCTTGGGTTGTAATTCGGAATACTAGTCGACCATCCGGTTCCGATTCCT20.11331444759206798No Hit
AATCAATAAGTGTACTTCTTAGCCACCTGGTCGCCCAGAGTGTTGATCTT20.11331444759206798No Hit
TTATTGTCCTGTAGCATATCAGTGATATTCAGAAGCCTCGGATGCTTGAT20.11331444759206798No Hit
CTCTTCCAGGTCCTGAATAGGCCCTGTCGAAGTACTTTGACTGACACCCT20.11331444759206798No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCATAGG20.11331444759206798No Hit
GTTAATTTTAATTGTATCTAGGTATATTTTCTGAATTTTCACTTCGCTCA20.11331444759206798No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1350.0087086288.59259395-99
Produced by FastQC (version 0.11.2)