FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg25361.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg25361.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408068
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24710.1754886837851581No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC4500.040.267056145
GCTGCCC5200.030.664911145
CTGCCGC15550.026.102373145
GTATAAG7300.023.8385851
GTGTATA8550.023.7456461
ATGCTAC3253.9875886E-822.3104691
CTGTACG6850.020.106999
ATCCTAT6150.020.0431441
CCTCTAT47550.019.0565199
GTTAAGG8500.018.767041
GTCCTAG6201.8189894E-1218.7120061
TCTATAC7050.018.5103133
ATCCTAG4404.8645234E-818.1272541
CCCTAGA4003.5641096E-718.1259692
CCCCTAG17550.017.7657431
ACAGTGC4908.625648E-917.7528958
TTGACCT59700.017.4852645
CCTAGAC4155.247366E-717.4695723
ACCTCTA52350.017.3092178
ACCTTTA12250.017.1611358
Produced by FastQC (version 0.11.2)