FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2535.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2535.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959690
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38940.19870489720312906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC7000.033.133636145
GCCGACC5950.031.671856145
CTGCCGC19550.028.917782145
GTATAAG11050.024.9347341
CTGTACG10950.023.8308099
TAGGACA11150.022.1048554
CTAGGAC10600.021.885173
ACAGTGC7450.020.4310768
TTATACT18600.019.0970734
CTATACT7050.018.5083224
TCTATAC10600.018.4656123
CCTTAAC11000.018.4531783
CTCTAGG12700.018.2696291
GTGTATA14200.017.8715881
CCCCTAG16450.017.6310231
TACTAGG8650.017.6011982
CACTAGG15700.017.5496061
CCATACC7450.017.516822
TATACCG5001.1084921E-817.3973775
AGGCCGT3753.874109E-617.396496
Produced by FastQC (version 0.11.2)