FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2534.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2534.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194915
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42070.19167029247146244No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC34150.15558689060852013No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG28030.12770426189624653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC8850.027.026445145
CAATGCG16200.025.9531789
CTGCCGC27750.025.596544145
CCCCTAG35950.025.4183431
CCTAGTG35050.025.2341693
CCCTAGT37250.024.3304652
GCCGACC9750.023.788311145
CCCTGTG16100.023.4176962
CGTTATG14150.022.5513111
GTGTATA14800.022.5409221
AGTGACC47200.021.8084096
ACAATGC21450.021.6277078
TAGTGAC47350.021.5867185
CTGTACG9100.021.5079989
TATACTG15050.021.1935485
CCTGTGA17850.021.1194343
CTAGTGA48300.020.8624384
GTATAAG12100.019.778991
TACACAG11950.019.4119685
CCTCTAT82250.019.38949
Produced by FastQC (version 0.11.2)