FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2533.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2533.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567697
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26280.16763443446023052No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC4850.035.861633145
CTGCCGC17000.032.82482145
GCCGACC5850.032.209057145
GTATAAG8700.029.1715031
GTGTATA10150.026.4329511
CCCCTAG23200.024.0664881
CCTAGTG22850.023.7981643
CCCTAGT23900.023.66352
GGACCGT1952.6189428E-422.3035586
GTGCTAG2205.898823E-419.7760051
CCTATAC4102.167144E-819.4525813
TAAGGCG2256.8771536E-419.3303685
GCCGTAA3001.1100858E-519.325436145
TATACGG4954.7475623E-1019.0374855
TAACACT9250.018.8103284
CTGTACG4654.5092747E-918.7074059
TATAAGA14900.018.4918172
TAGTGAC32850.018.0946375
CTTAGGC20650.017.9068223
AGTGACC33000.017.7921586
Produced by FastQC (version 0.11.2)