FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2532.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2532.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017876
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16880.1658355241699382No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC3900.037.166702145
CTGCCGC12650.034.375526145
GCCGACC3450.031.510899145
GTATAAG5400.030.878111
GTACTAA2951.4288162E-824.5750641
CCTTTAG2451.9129548E-623.6723041
GTGTATA6800.023.454731
TATACTA4607.2759576E-1222.060885
AATACCG3655.697075E-921.845025
ATAGCGC2003.1072157E-421.7457248
GGGGTAC6700.021.6375376
TATGGGT5750.021.4305694
TAGGACA5500.021.0867634
ACCTTAG1750.00365981520.7132681
CTATGCA2857.1102313E-620.3468789
TGTATAA7200.020.13792
GTACTAT3601.1767224E-720.13791
TATACCG2556.968157E-519.898055
CCTTATA3651.3609133E-719.8620362
GTACCCT2205.9112837E-419.768844
Produced by FastQC (version 0.11.2)