FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2531.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2531.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550293
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33500.21608818462058463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC5850.038.402607145
GCTGCCC7000.032.09361145
GTATAAG9500.029.0074231
GTGTATA12000.024.7771741
CTGCCGC13500.022.546047145
GTCCTAG4850.022.4284211
ATAGCGC2953.8881626E-722.1121718
TCTGACC9700.021.66612145
GGACCGT2704.4575117E-621.475896
TGTACAC6250.019.721233
ACCGTGT3701.5760634E-719.5888618
TGTATAA13850.019.3719372
CCTAGAG7250.019.0011843
CCCCTAG21650.018.7576871
CCTAGTG19000.018.6985343
CTATAGT5251.036824E-917.9569781
CTTAGGC28600.017.4923483
TATAAGA17000.017.4886422
CCTCTAT64700.017.3652239
TACCCTC3804.3938053E-617.167695
Produced by FastQC (version 0.11.2)