FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2530.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2530.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486597
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC20380.13709162604256567No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16460.11072267736313204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5100.034.10865145
GCCGACC6050.031.148806145
CAATGCG16250.029.8874389
CCTAGTG36600.029.3121643
CCCCTAG40000.029.0011331
CCCTAGT40300.027.881012
AGTGACC48350.026.6855536
TAGTGAC47850.026.3593435
ACAATGC20650.025.9755238
CTAGTGA49600.025.7216154
CGTTATG13100.024.9055521
CTGCCGC14300.023.315527145
GTATAAG7800.023.2380871
CCCTGTG12400.022.7994732
ACGTTTC23750.021.059027
GACAATG24950.020.9177327
TTATGAC18600.020.2655163
GTGACCT59250.020.0635687
TAACGTT14150.019.9790765
TGACCCC48000.019.9335828
Produced by FastQC (version 0.11.2)