FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2529.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2529.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249314
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCGC10300.037.289505145
GCCGACC5050.033.005356145
GCTGCCC5000.031.886044145
CTGTACG3902.0008883E-1124.160939
GTCCTAG7750.022.456171
ACCTTTA9700.022.4164588
CAGCGCG1700.003097483221.3184689
TAGGGCA2806.1047995E-620.7093689
TCTGACC9500.019.833426145
GTATAAG6800.019.1950741
CTATTTG49800.018.492711
CTATACA5901.2732926E-1118.430044
CTAAGGG2801.4277235E-418.1228753
CCTTTAT11250.018.0401619
CTTAGGC24650.017.9390723
CTAGGAC7450.017.5147253
TTGACCT55100.017.4972485
GTATTAG5001.1024895E-817.4035321
TTAGGCT27400.017.1968884
TAGCACC2550.001592544117.0568224
Produced by FastQC (version 0.11.2)