FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2528.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2528.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2510147
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30000.11951491287163661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC9200.036.234547145
GCTGCCC9350.034.1031145
CTGCCGC19500.027.872728145
GTATAAG13950.024.4311981
CCCCTAG32600.023.133261
CCTAGTG30750.022.3977493
CCCTAGT34150.021.8670252
GTGTATA19100.020.5013051
GTCCTAG12650.020.0631281
TCTGACC16700.019.961546145
CCCTTAG5900.019.664781
CTGTACG9200.018.1219679
TAGTGAC43700.017.9149785
CCTACAC6553.6379788E-1217.7094293
TACACAG14750.017.6923055
GTATTAT18200.017.5308281
CTAGTGA45950.017.5120664
CCTCTAT63750.017.3970879
TATAAGA22600.017.3232152
CCTAGAG16050.017.164633
Produced by FastQC (version 0.11.2)