FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2527.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2527.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307758
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23340.17847338727807438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5200.043.215717145
GTATAAG6100.039.2298771
CTGTACG9400.024.6786929
CCCCTAG15300.023.2240281
CTGCCGC14050.023.21772145
CTTAACT10450.022.1990134
TACCCCA4006.91216E-1021.7472635
CCTTAAC9700.021.6758463
CCTAGTG14750.021.1361683
CAATGCG7550.021.1239789
GAACCGT4851.6370905E-1120.9244026
GTAATAC4901.8189894E-1120.7148483
GTGTATA9500.020.6097641
CCCTAGT16450.019.8348832
TAGGCCC1850.00505024619.5928789
GGCTTAG17000.019.1953681
ACCGAGG2659.367018E-519.147078
GTTAAGG11800.019.0507721
GCCGACC4954.7475623E-1019.03801145
TCTGACC11050.019.024755145
Produced by FastQC (version 0.11.2)