FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2526.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2526.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993544
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20880.21015677212081196No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC4200.044.87263145
GCTGCCC3100.042.08897145
CTGCCGC8250.031.630499145
CATACCC3153.45608E-1127.6167073
CTGTACG4250.025.584789
GTATAAG5350.023.0400711
CCCCTAG10500.022.7883721
CCATACC3706.6484063E-921.5543982
ATTGCGC2053.6710105E-421.2155788
CCTAGTG10100.020.8150673
AACCCGT2806.0946695E-620.712536
CCTTAAC5403.6379788E-1220.1371823
TATGGGT4702.3646862E-1020.0514934
CCCTGTG3651.3598947E-719.863332
CCCTAGT11000.019.7730432
GTGCCTA1850.00505079719.5919489
CCGCGGA1900.00589245419.076379
GCTTAGG19450.019.0105882
TATAAGA10300.019.0051572
TAACCCG2359.205253E-418.509075
Produced by FastQC (version 0.11.2)