FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2525.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2525.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCAAATGTTTTAGACTCTATACTTACTTCAACGCGTATCACTAATTC11.8518518518518516No Hit
GATATAGATTGTAACTTTGATCCGGTAGTCAAACATGCATGAAACAACGC11.8518518518518516No Hit
TGCTGGCTCTATCTCGTCAAAAAACTCGAGAGTTGCGTCGTATTTGTCAG11.8518518518518516No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCATTCTCATCCATAAACATGACTATC11.8518518518518516No Hit
CTCCTGAGCAACATTCAATTTCTTTCGTTCATTCGGAAGATCTTCATTCA11.8518518518518516No Hit
ACACAGAATATAACAAATATTAACAATCAATTTATCAAGATTAAACGAGT11.8518518518518516No Hit
AATTTAATAGATCATATGACAAATAAAATAATTGTCATAAAAATAATTTT11.8518518518518516No Hit
TTATATGGAGCCAACATGTTTTTATTCTGGAGAACGACTACTTTCGAACC11.8518518518518516No Hit
GAATTTGGGAGAATGGTCGTTGACTACGATACAGAGAATAATCTGTCTCT11.8518518518518516No Hit
CATAGTACTCCTCTCTAAGAAACACACCCAGCACTCACTATTATACAAAA11.8518518518518516No Hit
GGTATATAGAGTTTATTCTAAAAATCCAGAGTTTTTCAAGTCTCAGCAAA11.8518518518518516No Hit
GTCTGAATCCGAATCGTCGAATGGATTCCAGTGACTTTCAATCAATTCAA11.8518518518518516No Hit
CTATTTGACCTCTTTTTGCCCTCTATTTGCCCTCTATTTGAGCTCTATTT11.8518518518518516No Hit
GAAGAGGAGACATGCCAGCATTTCTGGTAATCATCGCTCAGCAACGGCAC11.8518518518518516No Hit
CTGAAACTCAGACTACTTTTTCTATTAAAAAAATGACCCTTTTGAAAAAA11.8518518518518516No Hit
GTTAATCATATCGTAACCTTGCATTTAATTGATTCAGGTCCACAAAATGA11.8518518518518516No Hit
TTCAATATGTTGGAAACTTATGCATCAACTCTGGAAGAAGAAGTCTCTGA11.8518518518518516No Hit
TTCTCTCTCTCATCGTCCAATTTATTGTCGGATGTGTGTGTGCGGGAAAG11.8518518518518516No Hit
CACATAATGATGACCCTGAACCCCGATGACAAACAGATTGGATGAAATTC11.8518518518518516No Hit
AACCAGGATCGCTCACCACTAGACTATGGAGGTCGGACATCTGAGTAGGT11.8518518518518516No Hit
AAAGAGTAGAGCACAAGTGACCACTTGATAGCGCTAATGTCCCCAATTCC11.8518518518518516No Hit
GTATAATTCTGCGCTAAACGTGAAAGTTTAGAAATGAATAATATGTCTCT11.8518518518518516No Hit
CAATAAAACACGACATCAAATTACTAAAAAAAATAAAAATACATAGAAAA11.8518518518518516No Hit
ATAAAATAAACATAAGAAGAACAATACTAATAATAAAGTCTATAAAACAC11.8518518518518516No Hit
GGTTTCAAGGGGGCGGAGCCTATTTAAAAGAATTTTTATAGGATGTCTCA11.8518518518518516No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.8518518518518516No Hit
GATTCGGGAAGAAGATGGCCCGTCTCTCCTCTCTTGATCCTCTTCCTCCT11.8518518518518516No Hit
GGGTTAAATAAGAATGTCTCGAAATCATAGATCTGGACGAATTGAGGAAC11.8518518518518516No Hit
CATAAAAATACTAAACACAACCAACAGACAACAACAATACTATCAAATAG11.8518518518518516No Hit
CAATAAAAAATCGCCCGAGTCATTGTAGTAGAAAATGGAGAAACGGTCTG11.8518518518518516No Hit
ATATCAACAGAGAAAAGATACTAGCCCACACCTGCCTTCAGATAATTAAA11.8518518518518516No Hit
GAACTGGATATCATGTACAGCTTTAGAAATTACAACGTTTTGTATTATCT11.8518518518518516No Hit
AAATCCTCCAGTTCAAGAACAGAACGGAGAAGCTTCTCCAGAAGACGTTC11.8518518518518516No Hit
GGTTTTAAGCCGGTCAGAATCTGGAGGGAGCTTTTTGGAGGGGGGAGGGG11.8518518518518516No Hit
CATCTGCCGCTGCCGCCGATATGCAGTGTGTAGCTCTCGGTGGTCGCCGT11.8518518518518516No Hit
CATGTGAATTACTGTAGTGGGTGTTTAAAGGCGCGTAGCTTCTTTCTTGA11.8518518518518516No Hit
AAACATTTGAAATATATAAATATAAACTATAAAAGAAATAAGATACAAAA11.8518518518518516No Hit
AAAATAATCACAAAAACACAAATTAACAAATAAATAACAACAACAACAGC11.8518518518518516No Hit
ATAATACATTTTGAATGGATAGAAAAATGTAATCGTGGATGAATTTTGGG11.8518518518518516No Hit
GACAATGTTAAAATTTTTGATAACAACAAGTAAAAGTTCACGTTAACTAT11.8518518518518516No Hit
ACAAACTATAAGTATCAAACAAAGAACAAAACACAAATTATTTGAGCTCT11.8518518518518516No Hit
TGTTCGATCACGCAGAAAGAAGATATCAAAACACTTTTTTTCGGTAGCGG11.8518518518518516No Hit
ATAAAATTATATTTAAAAAAATATCACCAAGAATTAACAAAAAATTTATA11.8518518518518516No Hit
CACCTCGCACTCCTCTCTAGTACACCAACCTCTAACTCCAATCAGTCAAC11.8518518518518516No Hit
CATCTGAAGATGACGACTAATACAGATGTTTAGATATAGGTGATAGACGT11.8518518518518516No Hit
GATGGAGAGTTTAAGAGTTTTTGATATTTTGGGATATTTGCAGCATCGAG11.8518518518518516No Hit
TAAAAAAACGTATGCCATCTTCTCCGCGAGCATCGATTATACAGGCCACC11.8518518518518516No Hit
GAATATCACTCTGAATTGGTTATGTAACTTGAAAGCGTTTCACGATCCGT11.8518518518518516No Hit
TTTTTCTTGAGTCCTTGAAACCAAGGGGAAAATTATTTTTGTGCAGCCGG11.8518518518518516No Hit
GTTTAGAGCATCGAGGAATTCATTGGCCGTCCTAATTTCGTGATCTCTTC11.8518518518518516No Hit
CGCAAACCTTGTCCAGGAACGGATCCTTGTACCCTTCGAACAAAAGCTCT11.8518518518518516No Hit
CCCTAGCCGTGGCTAAGGTTGAAAAATAACTTGATGATTCAAAAGAGATC11.8518518518518516No Hit
ATGATGAAGATGATTAATAATATGAGATATGTTTAAAAAAAAAATTATTC11.8518518518518516No Hit
CATCTGACGCTGCCGACGAAGGCTTAGGTGTAGCTCTCGGTGGTCGCCGG11.8518518518518516Illumina Single End PCR Primer 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGC50.0145.08
CAATAAA50.0145.01
ATAAAAA50.0145.03
AAAATCG50.0145.07
GATTAAA50.0145.0145
AAAAATC50.0145.06
AAAAAAT50.0145.05
AATAAAA50.0145.02
AATCGCC50.0145.09
TAAAAAA100.072.54
Produced by FastQC (version 0.11.2)