FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2524.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2524.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144705
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36570.17051296098997298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC7700.036.708458145
CTGCCGC22250.028.3388145
CTGTACG8350.027.7809359
GCTGCCC7500.025.124897145
GTATAAG11650.024.8959581
GTCCTAG10600.023.257761
CCTTAAC9550.022.0155053
CCTAGAG12900.021.3563943
TAGCGTG2556.966468E-519.9003855
CTAGGAC10900.019.2888153
GTATATA13700.019.0535871
CCTAGTG16900.018.8755663
GTATTAG7000.018.6452941
TATACCG5158.058123E-1018.2995225
GTGTATA13550.018.1942621
CCCCTAG18150.017.9775581
CCCTAGT18450.017.683592
TCCTAGA17100.017.3836942
CTTAACT13800.017.3359894
CCCCTTA16000.017.2211
Produced by FastQC (version 0.11.2)