FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222893
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24940.20394261803771876No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC16020.13100083163449294No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC3800.040.05127145
GCTGCCC4950.033.674664145
CTGCCGC14500.029.989132145
GTGTATA7750.029.9368781
GTATAAG5850.029.743763
TGTATAA7900.026.6140732
CATACCG3108.60382E-1025.7227945
CCCCTAG15450.023.9331511
ACGCGTT4600.023.632738145
CCTAGTG15600.023.237313
CCCTAGT16400.022.9879362
GTCCTAG6450.021.3575821
CAATGCG8100.020.5840119
AGTGACC22500.020.2976256
TAGTGAC22200.020.2453775
GTATAGT5453.6379788E-1219.9550571
GTATTAT8500.019.618561
CTAGTGA22700.019.4816934
CTGTACG4904.1472958E-1019.232449
ATAACGT8000.019.030584
Produced by FastQC (version 0.11.2)