FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2520.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2520.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545695
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34330.22210073785578657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC7650.038.84462145
GCCGACC6700.037.861835145
GTATAAG8900.032.589311
CTGTACG8750.026.5149549
GTGTATA9300.025.7297861
CTGCCGC17750.025.316391145
TGCTACA4053.274181E-1123.2729432
CTTAGCC4607.2759576E-1222.0650583
GTTAAGG10950.021.1904931
GTGCTAA3457.498784E-821.0177441
GTATATA10300.020.415781
GTCCTAG3951.4108082E-820.1929971
GCCCTAG1800.004304262320.1420041
ACAGTGC5505.456968E-1219.7725878
CCCCTAA5555.456968E-1219.5976281
TCTTACC10750.019.552305145
CTAGGAC5204.3655746E-1119.519093
TGTATAA11150.019.5078492
GTATACG3751.8068204E-719.3363251
GTATTAT9500.019.08191
Produced by FastQC (version 0.11.2)