FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010588
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41560.20670570002407257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC7350.029.585648145
GCCGACC7450.028.215574145
GTATAAG10100.026.561781
CTGCCGC18850.024.225702145
GTGTATA12300.023.5793441
TCTGACC12650.021.774101145
CTGTACG8850.021.296579
GTCCTAG10500.020.02561
GTTAAGG10700.019.651291
GCTTAGG26350.019.5329632
GGCTTAG26900.019.1373981
CCTAGTG28850.019.0976453
CCCCTAG29650.018.8296761
GTCCTAA5851.0913936E-1118.5914061
CTTAGGC26900.018.326043
CGTTATG12200.017.8294621
CCCTAGT31450.017.7484512
GTATTAT13650.017.529041
CAATGCG13350.017.375949
CATAACG10450.017.3434583
Produced by FastQC (version 0.11.2)