FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923724
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5900.047.912483145
GCCGACC7100.037.772827145
CTGCCGC18800.031.61492145
GTATAAG10950.029.7968221
CTGTACG7350.022.682939
GTGTATA12850.022.0055921
CCTAGTG24750.021.08873
GTCCTAG7650.020.8512841
CCCCTAG25750.020.8365631
CCCTAGT26750.020.0555382
GTCTTAG4752.728484E-1019.843641
CTATACT6700.019.474564
CAATGCG12550.018.482689
CATCTGC18100.018.42109145
CCTAAGC40950.018.4122072
CTACACT6700.018.392644
GCCTAAG43350.017.5619131
CTAAGCC43550.017.4780773
CCTCTAT42750.017.4646459
AGCCTAA44300.017.344016
Produced by FastQC (version 0.11.2)