FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992096
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26250.13177075803575733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC7050.033.924316145
GCCGACC7700.033.884262145
GTCCTAG5600.027.1916521
GTATAAG10700.026.429271
CCCCTAG32600.023.7996481
CCCTAGT32450.023.4586282
CCTAGTG30300.023.4477163
CTGCCGC17950.023.418001145
CTGTACG10450.021.5034439
CTACTAG4904.129106E-1019.2376331
TCTGACC12450.019.210154145
CCCCTTA25150.018.7404381
CCTAGAG10450.018.7311553
CCTTAGG3101.4682608E-518.7092462
CCTATAC6251.8189894E-1218.5591073
TAGTGAC39950.018.506035
CTTAGGC24400.018.4212443
CTAGTGA41750.018.4030634
GCTTAGG24200.017.969838
GTGTATA11300.017.9673461
Produced by FastQC (version 0.11.2)