FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2515.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2515.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100181
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46420.2210285684900492No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC7650.037.898212145
GCTGCCC8700.037.489826145
CTGCCGC19950.027.248245145
GTATTAG9200.022.071321
GTGTATA14550.020.4352041
CCCCTAG24500.020.1280141
CCTAGTG23100.019.1466673
CTGTACG9250.018.8097389
CCCTAGT25350.018.3070642
GGCTTAG47850.017.429061
CTTAGGC47150.017.3768923
GCTTAGG47350.017.3051412
GTATAAG11050.017.0602873
TTAGGCT51000.016.9157034
AGGCTTA48750.016.802576
GCCTAGC2600.001809278416.7353951
CCTAAGC51200.016.7120112
TAGGCTT51450.016.6260555
CTAAGCC52700.016.5099563
GTTAAGG11450.016.4674341
Produced by FastQC (version 0.11.2)