FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2513.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2513.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3867428
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45170.11679596879373062No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC39060.100997355348309No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAG70500.027.3601861
GCTGCCC21500.026.96654145
GCCGACC22750.026.759104145
CCTAGTG70500.026.529773
CCCTAGT72450.026.2183362
TAGTGAC94800.022.6335435
CTAGTGA96600.022.286844
AGTGACC98500.021.9299666
ACAATGC40650.020.8627728
CAATGCG37300.020.794569
CCCTGTG26550.020.4804252
CTGCCGC53250.020.142612145
CCTGTGA29000.018.7484823
GTATAAG19750.018.358171
CACAACG23850.018.2375183
ACGTTTC52900.018.223727
CCTCTAT104900.017.552269
CGTTATG32450.017.430361
GACAATG51300.017.2378677
GTGTATA22050.017.1009881
Produced by FastQC (version 0.11.2)