FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2510.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2510.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380426
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23370.16929556528202164No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5600.040.117134145
GCCGACC6350.038.802654145
GTCCTAG3800.030.531241
GTATAAG7550.027.852171
CTGCCGC16100.027.457449145
GTGTATA8700.022.5036281
ACAGCGT3106.2595973E-721.0441558
TCCTAGA7700.020.7153742
CCTAGAG4901.8189894E-1120.712373
CCCTAGT19500.020.0779782
TAGCACT4702.382876E-1020.0513364
CTCTAGG6200.019.8822381
CTAGGAC4752.728484E-1019.8402713
CCCCTAG19200.019.6385821
CCTCTAT47800.019.5626729
TATACCG3351.3167737E-619.4751055
CCTAGTG17250.019.3315453
TACCGTG3502.004048E-618.6391077
CAATGCG9900.018.3050619
ACCTCTA50700.018.3000668
Produced by FastQC (version 0.11.2)