FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2509.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2509.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240191
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22110.17827899089736984No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC17680.14255868652489817No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG14940.12046531542318885No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC4800.037.749916145
GCCGACC4850.034.371883145
GTATAAG5800.030.0049671
CCCTGTG6950.028.1667212
CCTAGTG19550.026.328973
CTGCCGC14050.026.309408145
CCCCTAG20250.026.140131
CAATGCG7900.025.6921639
CCCTAGT21100.024.7404382
TAGTGAC24200.023.3631535
GTGTATA7450.023.3595711
AGTGACC24800.023.091136
CTAGTGA24050.022.6083324
ACAATGC10750.022.2532148
CCTGTGA9050.021.6290533
GTGACCT29450.021.4134277
CGTTATG6600.020.8746661
TAAGACC3651.3613317E-719.8622954
CTGTGAC10300.019.7080254
ACTGTGC3701.5725163E-719.5923048
Produced by FastQC (version 0.11.2)