FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2508.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2508.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3880399
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96030.2474745509417975No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC13900.035.977123145
GCTGCCC14100.035.466805145
GTATAAG19250.030.5151231
CTGCCGC44650.027.107357145
GTGTATA22200.023.5201631
CTGTACG12550.021.949069
GTCCTAG13000.020.0826031
TCTGACC26350.020.078644145
CCTAGAG16750.019.9101753
TTATACT29150.019.8960424
TATAAGA35150.018.9767822
CCTTAAC14650.018.8052023
CTAGGAC13700.018.5216453
CTCTAGG16200.018.3539561
GTATTAT28050.017.839271
TAATACT26100.017.4990844
TGTATAA30300.017.4678842
TAGGCTT61150.017.4270945
TACACCG6303.6379788E-1117.2605575
GCCTAAG61900.017.2221571
Produced by FastQC (version 0.11.2)