FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2507.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2507.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808894
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9990.1235019668831763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTAC1551.4649922E-632.7406653
GTTAAGG5550.030.053171
GTCCTAG2202.257184E-829.667111
CTGCCGC5450.029.25949145
GTATAAG4300.028.6705441
CTGTACG5000.027.5472289
GCCGACC2601.1466727E-725.090527145
CCCATAC3501.2914825E-1024.8638671
GTATAGG2401.5936948E-624.1732031
CCCTGTA1801.5196975E-424.1672232
CCCCGTC1500.001495440724.162747
GCTGCCC3301.7753337E-924.161247145
AGACCGT1851.8330298E-423.508246
ACCGTCC2502.2815402E-623.1962328
GTGTATA4403.6379788E-1223.0744191
TGTACGG1902.1941599E-422.8924335
CACCGTG1600.00217684722.652577
GTTAGAC2252.6515263E-522.5546823
CATACCC3905.020411E-1022.3068263
ACACCGT2603.2091484E-622.302696
Produced by FastQC (version 0.11.2)