FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2505.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2505.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399019
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23330.16675970805257112No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC15280.10921938872881641No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC6200.037.40895145
GCCGACC5500.027.674122145
GTATAAG6250.026.6783033
CTGCCGC14850.024.892067145
TCTGACC9350.024.03075145
CCCTATG2852.7811802E-722.89412
GTGCTAG2205.9003977E-419.7750051
CCCCTAG9900.019.7750031
CTTAGGC29600.019.348433
AGGCTTA30450.019.2824176
TAGGCTT30650.019.1565955
GTCCTAC2659.344809E-519.1531491
CTGTGCG2307.9751824E-418.9091369
GGCTTAG29950.018.8836421
CCGCGCC2701.0812731E-418.7924149
TTAGGCT31700.018.751414
CACAGTG6700.018.3910647
GCTTAGG30650.018.2131962
AGTACCG3602.6224752E-618.1219045
GTGTATA8450.018.0198271
Produced by FastQC (version 0.11.2)