FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2504.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2504.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2667828
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56360.21125799714224452No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC9750.034.94042145
GCCGACC8300.032.311604145
CTGCCGC26750.027.90926145
GTATAAG13100.026.5675431
GTGTATA16150.020.6522181
TGTATAC12950.019.5936133
GTCCTAG6301.8189894E-1218.414541
TCTGACC17900.017.816996145
TATACCG8150.017.7901885
TACACCG4908.629286E-917.7538815
TATAAGA24850.017.504812
CCTAGTG33500.017.3125653
GTATATG14700.017.263631
GTATTAC10400.016.732441
CCCCTAG34300.016.6999191
CTGTACG6607.4578566E-1116.4755219
CTTAGGC54600.016.4643763
GTATTAG11050.016.4043541
TTAGGCT59500.016.3266074
CCTAAGC70650.016.110912
Produced by FastQC (version 0.11.2)