FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2503.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2503.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031728
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41220.20288148807320666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC6950.039.627743145
CTGCCGC20300.035.702988145
GCTGCCC6350.030.817019145
GTATAAG8550.029.687241
ATGCTAC6400.027.1956331
CCCTAAG2202.2251183E-523.075081
CCCCTAG23150.022.8686081
GTCCTAG9650.022.5456031
CCTAGAG11400.020.9874343
TGCTAGG2455.1113762E-520.7174192
CCTAGTG23300.020.2258933
GTATAAA17300.020.1216241
TGCTACA6850.019.0539782
CACAGTG6500.018.9578727
CCCTAGT24350.018.7605382
GTGTATA13200.018.6798291
ACAGTGC7050.018.507068
CTTAGGC35100.017.9705923
AGGCTTA36350.017.946576
TAGTACA6950.017.7325654
Produced by FastQC (version 0.11.2)