FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2502.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2502.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3230891
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70740.21894889056919592No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC11700.042.126247145
GCCGACC10500.041.418243145
CTGCCGC34650.027.612164145
TCCTAAG6300.026.469852
GTCCTAG17900.025.9245571
CCCCTAG43150.025.3735081
GTCCTAA6700.024.8907181
GTGCTAG5250.024.8597971
CTTCCTA19950.024.3509711
TGCTAGG4550.023.9025422
CCTAGTG42300.022.9661563
CCCTAGT44300.022.749682
CCTTAAC15000.021.2659743
CTGTACG12700.021.1184189
CCCCTAC5600.020.7164971
TAGGACA20800.020.5626434
CCTAGAG22250.020.5274223
CCCTAGA9700.020.1816312
TGCTACA11700.019.8302562
ATCCTAC8900.019.5526491
Produced by FastQC (version 0.11.2)