FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274066
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC21240.166710358804018No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18490.1451259196933283No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG17790.1396316988287891No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC4100.035.358368145
GTATAAG6200.031.5770511
CTGCCGC14700.026.627014145
GTATTAG5150.025.3433951
GTGTATA9900.023.4376621
CCTAGTG16800.021.5745093
GTTACAC3706.664777E-921.5511843
CCCCTAG17400.021.253011
CCTCTAT39250.021.2408779
CAATGCG10250.021.2183519
ACAATGC13900.020.862178
TATAAGA11550.020.7147792
CAGCGCA2505.9786584E-520.298899
ACCTCTA43050.020.2079548
CGTAAGG1800.004311030320.1362083
CCCTAGT18150.019.9729272
TAGTGAC21150.019.5364065
ATAACGT8950.019.4387514
ACCGTGC1900.00589061519.0779028
CATAACG8050.018.9105243
Produced by FastQC (version 0.11.2)