FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2499.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2499.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470308
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6790.14437347440400758No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG350.00356712162.1349753
GCACGAT350.003568624162.1283656
TGGCCCG350.003568624162.1283656
GGGACGT400.00605448454.362326
GCCCATG450.00964081648.32727
AGTCCGC450.00964081648.32728
TACTGGG752.550416E-548.32727
TATGGCG604.948078E-448.3220635
GGCACGA450.00964486848.322065
AGGGACG450.00964486848.322065
TATACGG1255.1968527E-946.3891835
GTATAAG2050.045.9844441
CTGTGCA657.3295E-444.6192179
GTCTAAC700.001055070841.4365351
ACCGTCT1053.8231174E-641.4233178
GGACTGC700.001057281941.418916
CTTCCTA1252.710749E-740.6078031
GCATTAG907.4679156E-540.285521
GCTGTTA750.001481185838.67411
GGTGTCC750.001483510738.6617628
Produced by FastQC (version 0.11.2)