FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2494.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2494.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2453448
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57470.23424176913470351No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC9300.042.081146145
GCCGACC8200.032.70127145
CTGCCGC22900.029.115824145
GTGTATA15950.022.7313441
GTATAAG12300.021.812851
CTAGGAC12100.020.9692733
GCCGTAA5555.456968E-1219.58732145
CCTAGTG26500.019.1492983
TAGGACA14450.019.062944
CCCCTAG27250.018.8932931
CCCTAGT27250.018.6237742
CTGTACG8900.017.9187139
TTAGGCT56250.017.7840524
TCTGACC16000.017.665314145
TCTATAC13200.017.5742473
GTACACG6303.6379788E-1117.2649971
CTTAGGC52350.017.17143
CATCTGC23550.016.925776145
TAGACAG12500.016.8171845
TAGGCTT55750.016.7729765
Produced by FastQC (version 0.11.2)