FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2493.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2493.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464988
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5560.11957297822739511No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG1001.3874836E-436.2625121
GTGTATA2800.033.6723331
TCTGGGC1153.155116E-431.5292243
GCCGACC2151.8131686E-830.336456145
GTACAGG1000.00606092829.010011
TTAACAG2252.8037903E-829.006893
CCTTAGT1000.006064116529.0068872
AGGTAGT1306.4850005E-427.8792366
CTAGACT1855.8112128E-627.4300924
GTATAAG2405.241236E-827.1968821
TACTGCA1358.085278E-426.846677
GTCTATA1658.389333E-526.3727361
GGGTAAG1100.00965011526.3727341
CCCTTAG1100.00965011526.3727341
CTTAGTA1100.00965517926.3698982
TGAACCG2301.1028424E-625.2125265
GCTGCCC1500.001496624824.156809145
CTGCCGC4303.6379788E-1223.59502145
GTATAAA3803.601599E-1022.902641
TGTATAA2852.7658098E-722.9001752
Produced by FastQC (version 0.11.2)