FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2492.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2492.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313547
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35330.15270923823894653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC8800.039.531696145
GCTGCCC9500.038.90751145
CTGCCGC27000.033.82156145
GTATAAG11350.026.1967371
GTATAGG6850.023.2911721
CTAGGAC13650.021.2462963
CCCCTAG29950.020.0974271
CTGTACG10250.019.8026969
TCTTACC15750.019.326607145
GTACTAA7350.018.7467271
GTCCTAG11850.018.3595521
TAGGACA16850.018.0700074
CTACCCT5751.8371793E-1017.6509934
CCCTAGT32550.017.5974922
GTGTATA13700.017.4683781
CCTAGTG29950.017.4297663
CTATACT8900.017.1055974
CCCTGTG11250.016.756972
CGTTATG13000.016.7354371
GTATATA18000.016.5184971
Produced by FastQC (version 0.11.2)