FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2490.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2490.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309794
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20280.15483350816998703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC4000.041.675995145
GCCGACC4600.040.96695145
GTGTATA7050.032.9163131
CTGCCGC15200.027.179998145
GTATAAG6250.026.6869011
CCTATAC3004.5575325E-721.7498113
GTACCGT3157.2939474E-720.7117336
TATAAGA10850.020.0451434
TCCTATA4904.1472958E-1019.2352622
TGTATAA9550.018.9796182
TTAGAGT5759.094947E-1218.9121574
GGACCGT1950.006846529418.5874526
ATACTGC4705.145921E-918.5083566
TCCGCAC2400.001060010718.1248423
CTATACT4857.588824E-917.9373044
ATGCTAG3352.8529697E-517.3179131
CCTAGAC4205.9473314E-717.2617553
ACGCGTT3804.39611E-617.166315145
GTACTAG4659.131509E-817.1549741
CATCTGC12750.017.054117145
Produced by FastQC (version 0.11.2)