FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1114.864864864864865No Hit
CAATATACTAAAAAGATCGTCATTATGTCTCCGTGAAAATTGAAATCGGG11.3513513513513513No Hit
CCCCTCCCCCTTTTCAACGTCTACTTATCGATCTCGACGTGAAAACTAGA11.3513513513513513No Hit
TTCCAGTGGGCCTGAAAATTTTGGGTGTCGTAAGGTCGGGAGTGGTCTTG11.3513513513513513No Hit
CCTCTCTCTTAACATTCCCGGAGTAGTAGGTTTCATCAATTCCTTCGGGT11.3513513513513513No Hit
ACCGGATACCCTGGTGAAGCGTGCCTCTTCTCCCGAGAACGATTCCGTGC11.3513513513513513No Hit
CACAAAAACACAATATTATTACATATATATAAAATAAATAAAAATATCTC11.3513513513513513No Hit
CATCGGTACTTTTTCGTCCTCAAAAAGTATTCAAAGGGACTAGACATATA11.3513513513513513No Hit
ATTGAATAGATGCCTCCCGTTTTTTTAAAATTTTATTGTTGAATAGTGAA11.3513513513513513No Hit
GTTGATAACAAACAGAAAATACTAATTTCTCATTGTGTAATCTGAATAAT11.3513513513513513No Hit
TATGATGCTCTTCTGTCCAAACATAACTTCACTCTTGTGAACCGCAAACT11.3513513513513513No Hit
GGCATGGGGAGGCTTGGGAGGTGGGTAGCGGACAGGTGGTTGGCATGGGG11.3513513513513513No Hit
CTTCCACCCCAATCACATCGACGTTTTCAAAGCGGTTTCTGAGCTGTCTC11.3513513513513513No Hit
CAGATACACCAACCAGCTCAAGCTCTCCAGACGATACCTTTATCCCTGTC11.3513513513513513No Hit
TATTTATGGTTTGGAGTGGGTCTTTGGGGGATGGGTTTCGATTTGGCAGG11.3513513513513513No Hit
GTGTGAATGGGGTGCGCTGACAGTTGGGTGCGGGTGCGGACGGCGCGTGG11.3513513513513513No Hit
CTTGTTAGGCCACGGAGATTTGGAAGATTTTTTGGAGAAAACATTTTTTC11.3513513513513513No Hit
CACATGCATTGATATCGTGTTACAAACTCATCGTATCATTGACTCCTATG11.3513513513513513No Hit
AATCTGCGCCGAGCGGGAGGGTGAGACGCGTGCGGGGTGGGGTGGCGGGG11.3513513513513513No Hit
CAATAACACATCGAGAATACACGAATTTTTGATGTTCGGCCGTCTTTCAG11.3513513513513513No Hit
GTCATGACGGAGCTAGCTCAGTTTTGAGGTGTGCGCTACCGACGCGCCTT11.3513513513513513No Hit
GCGGTGGTGTGCGCAGGTAATAGGTATTTTTTGGACGTCGTGTATTCTAT11.3513513513513513No Hit
CTTTTAGTGTACTTGTGAGTTTTATTTCAAATGACGAAGTGGGCCTCTTT11.3513513513513513No Hit
GGGCGGGGGGGGGGGGCGGGGGGTGCGTGGGGCTGGGTGGGCGGTGCTGC11.3513513513513513No Hit
GCGTCTCTTCAGTTATCTCCATTGTCTAATCTCTATAATTAGAGGTCAGA11.3513513513513513No Hit
CATCTCACTAAAACTAACAAACAACATTATTATATAAATGATCTAGAATT11.3513513513513513No Hit
ACATACAACTAAGAAAATTAAAAAAAACAAAATACACACAAATATAGAAA11.3513513513513513No Hit
GATATGGTATGTGTCTTAATCTGATAGTTCAGTGACGTGTTGATATTCTA11.3513513513513513No Hit
GATATTGTCCGACTGTGGAGTGTTTGCCAGCCCTCTAGTGCCATTTAATT11.3513513513513513No Hit
CGCAGTCTCTCATGGGCCACTCAGGAGATGCCTCCCCCGTTAGCTGGCAA11.3513513513513513No Hit
GGAGTACCGTCCATGTCCCTATTGTTTAACTTAGCACTGAACTCCTCAGA11.3513513513513513No Hit
TCTAGACACACCAAAAAAATAAATCAAAAACTCACGTAAACCTACCTCTT11.3513513513513513No Hit
CAATAAACTTCTGAGCATTGAGAGTGACTGACTCCTTGTGCTGTCTCTTA11.3513513513513513No Hit
GTAGGGGTCTCTTAAAATGATTTCTCTTGCTCAAATCCTCTGGAATCCCG11.3513513513513513No Hit
TGTCCCGGGCGGGGGGCGGGTCGGCGGGGGGGGGCTGGGGGGGGGGGGGG11.3513513513513513No Hit
ATATAGAAAAGAACGAATAGAAATGGAAACATGAAAACTTACACAGAATC11.3513513513513513No Hit
CTGTTAAACCCAAGATAAAAATGCTGCAATCAAGAAAAACCACGACAACT11.3513513513513513No Hit
GTTATAAATCACCAAATAGGAATTGATAAAAATAAGCGAACACTTAGCAA11.3513513513513513No Hit
AAAGAACATTTGGGGTCACTTGTGGTCATTTGGGGTTGCTCTGTGTCTTA11.3513513513513513No Hit
CTATAGATCTTTTAAAACTAAACAATTAAACATTAACTTCTCAAAAAAAT11.3513513513513513No Hit
GGGGGAGGGTTTTCAGGGGGGGAAGGGAGGGGTCAAAGCGGACCGGGGGT11.3513513513513513No Hit
GCGTGGGTAGTGGCGCTCCGGCGGAGCAGAGGGGGCTGCCGGAGTTGACG11.3513513513513513No Hit
CACACACACAGAAATCATACGATTCCATGTTTTCATCATCATCATCCCCC11.3513513513513513No Hit
CGTGCAAACCGGTGTTAGAAATATTCACCATAGTGCTCTACTTTATCAGT11.3513513513513513No Hit
GGGTTGGTGTGGGGCGCGCGGGGGGGGAGGGGGGGGGGGGCGGGCGGGGG11.3513513513513513No Hit
GATGAATGCAAGTAAGGTGATTCCAGCGTCATATATTCGGAAAAAAATTG11.3513513513513513No Hit
GCTCGACAGTGATGATGATGCCTGGATTCCTCTCTATGCTCTTTTCGGTT11.3513513513513513No Hit
CTGATATCAAATCAAAGACAATTATATTGAGCTTAAAGACACCAAGAAAA11.3513513513513513No Hit
CTCCACGTGTTGCTCCGTCGTGCGCGTGCGCGATCATTTGCCATCTGATT11.3513513513513513No Hit
ATATTCCCCAGTTATTATATCTTTCCATTCGAATTCATTCTCTCTTTATT11.3513513513513513No Hit
ATTAAAAGACGTCTAAACTGGCGATGTTAGAATAGCTTTATGGATACAAC11.3513513513513513No Hit
ATAGAAGAAGACATCGGAGTGGAATTATCTCGAAAGGATCCCCTAAAAGA11.3513513513513513No Hit
AATAAATCAACAAAAAATAAAGATAATAAAATAACAAAAAAATAAAATAA11.3513513513513513No Hit
TGGTAGCGGAAAATTAAAAAAAAAATTTTATGCGCAGGATGATTAAAAAT11.3513513513513513No Hit
CTCAAGACTCAGTACTTAGGAATGGCCAGGATCCACTCTTTTAAGGGAAG11.3513513513513513No Hit
GCGGTTAGGCGGGTGCGGGATGGGTGGAGGGTTGGGTTGGGGTGGGCGGC11.3513513513513513No Hit
CACGTATAGTTAACTGACTGTTGAATTACACCCCCATCCCCTTATTCTAT11.3513513513513513No Hit
CTCTTGAACTCTCTCGTCAAAGTGCTGGGCAACTTTCCCGCACGGTACTT11.3513513513513513No Hit
GTAATAAGCCAAGCGGAAAGAATCATAAGTTAGATAGATTCAAGTATGCA11.3513513513513513No Hit
ATCTCATGATTATACTTTTTCGAACCGGAAAAACAAGCTAAATTCAAATC11.3513513513513513No Hit
AGAAAAAGGAATCATTCCTCTTGTTGGTGGCATCGTCGAGGCACAACCGA11.3513513513513513No Hit
AAAAATTGGTGAATTTGTGTGATGAATTTAGGGAAAACAGAGAGAAAAAG11.3513513513513513No Hit
AATTATTACCATAAGTTTTAGAAACTGGAATAGAAAAAATGAATTTATCC11.3513513513513513No Hit
TTTCCTGACTGGGAACCACTATTCCAATGGATGTTTACAAGATGCTGCTG11.3513513513513513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATCG50.0145.07
ACATCGA50.0145.08
ACACATC50.0145.06
ATAACAC50.0145.03
CAATAAC50.0145.01
TAACACA50.0145.04
AATAACA50.0145.02
AACACAT50.0145.05
CTCTGTC50.0145.0145
CATCGAG50.0145.09
Produced by FastQC (version 0.11.2)