FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2486.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2486.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655034
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7870.12014643514687787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC3150.041.42262145
GCTGCCC2705.456968E-1232.217594145
AGTACCG1000.00607268129.000265
ACCCTAG1050.00770333327.6214031
CCTAGCC1050.00770620427.6192953
CTGCCGC6800.026.65058145
TACCAGG1100.00966877326.3638734
GCTACAT1658.4137064E-526.363873
GCTGTGC1100.00967237326.3618588
CTATACT1958.747853E-626.0258754
GGTTATA1851.8286692E-423.515521
CTGTCCC1550.00180853523.385529
TCTGACC5050.022.966995145
GTACTAT1902.1907796E-422.896691
CTCCTAT2552.7027108E-622.7470381
GTTAAGG1952.6118997E-422.3095971
CTAGGAC2303.141522E-522.063736
TAAGTAG1650.002599775821.9698935
ACCCTAT1700.003090139521.3253481
CTACTAG1750.00365559520.7160531
Produced by FastQC (version 0.11.2)