FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2485.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2485.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850399
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC27210.1470493661096877No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25760.13921321833831515No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG18920.10224821781680601No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGTG16400.034.4816632
GCCGACC7300.033.757954145
CTGCCGC17550.030.5615145
CCTGTGA19450.029.8192063
CACAACG12250.029.5909983
GCTGCCC6400.029.445127145
CAATGCG18250.027.4094419
CAACGTT14400.027.1859935
CCCCTAG41950.026.0986231
CTGTGAC23050.025.7896294
TGTGACC28100.024.7673115
ACAACGT14950.024.7304044
CGTTATG15900.024.6246051
ACAATGC21850.024.5510888
ACGTTTC30150.024.2848997
CCCTAGT44800.024.1126922
CCTAGTG42650.023.9676673
CGACCTC9150.023.7691766
TCGACCT9000.022.5543065
CCCCTGT25450.022.5067081
Produced by FastQC (version 0.11.2)