FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2483.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2483.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549885
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11330.20604308173527192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC1900.053.42233145
GGGACCG657.332829E-444.6164479
CTGCCGC4850.043.35155145
GTGTATA2551.8189894E-1234.124671
ACCCTAC900.003623168232.228851
CCTACAC1352.1401089E-532.2229923
CTGTACG2951.4551915E-1129.492239
GTTAAGG3850.028.2525671
GTATAAC1804.6873974E-628.2002471
AGAGTGC3102.7284841E-1128.0651848
CTGCTAG1306.471416E-427.8903521
CTACACT2354.2968168E-827.7615684
CCCTATG1050.00770458627.6197072
ACAGTGC2156.1148785E-726.9773888
TCCTAAC1358.076933E-426.8524932
GCTATAG1100.00966674726.3642643
CAGTGCA2207.4741183E-726.3642649
GTAGACC1409.998073E-425.8887634
GTGTTAG1450.001224270925.0051461
CTGTCCG1801.5188257E-424.1672429
Produced by FastQC (version 0.11.2)