FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2482.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2482.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391905
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21080.1514471174397678No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5850.047.074764145
GCCGACC4800.040.76458145
CTGCCGC12550.028.295202145
TATACCG3306.184564E-1126.3642275
GTATAAG7550.024.0123371
TGCTAGG2053.6677747E-421.2192232
CACTAGG17650.020.9539931
CTTAGGC29950.019.8494783
CTTAAGG2205.9054996E-419.7724593
AGGCTTA29850.019.6730986
GGCTTAG30850.019.5103151
GCTTAGG30150.019.4773482
TTAGGCT31850.019.3482464
TACCGTG3051.2760762E-519.0154517
TTATACT10700.018.9717044
TAGGCTT31400.018.9335445
ACTAGGG19150.018.9292452
GTATATA10450.018.7365151
TAACGTT6600.018.674665
CTAGGGG18150.018.3744033
Produced by FastQC (version 0.11.2)