FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2481.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2481.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256652
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36120.1600601244675741No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC8900.047.233818145
GCCGACC9400.032.384445145
CTGCCGC27300.031.328125145
CTGTACG6850.027.5153379
CCCCTGG3551.546141E-1024.5131441
GTATAAG8250.023.733181
GTGCTAG3452.9613147E-923.1216961
CCTACAC5250.022.094863
GTACTAA6300.021.8705221
GTCCTAG5051.8189894E-1221.5400141
CTAGGGT2704.4530316E-621.479214
TACTGCG3406.452137E-821.3198577
CCCCTAG24050.021.1071241
CCCTAGT24250.020.9297942
CTTAACT11350.020.4383684
CCTAGTG23850.020.3665283
ACTGTGC6150.020.0371998
CTACACT8900.019.5484944
CCTTAAC9350.019.3846953
TCTTACC16100.019.357872145
Produced by FastQC (version 0.11.2)