FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2479.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2479.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645959
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20190.12266405177771741No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5100.039.794765145
CTGCCGC15150.032.05533145
GCCGACC5850.023.541592145
TCTGACC10200.022.029245145
TAGGGTG3304.7044523E-821.9653115
GTATTAA8800.019.7747881
CCCCTAG11750.019.7467381
GTATTAC6800.019.1931761
GTATAAG7200.019.1339381
GTCCTAA4202.856541E-818.9900721
CTTAGGC21950.018.8242653
CCTAGTG11950.018.8049183
TGGGCAG4253.283094E-818.760965
CTGTACG5458.54925E-1118.6190039
GCTTAGG20600.018.3000972
GTATTGG8750.018.230471
TTAGGCT26000.018.1224824
TAGGACA7600.018.1224824
TAGGCTT24550.018.010665
CTAGGGG10900.017.956223
Produced by FastQC (version 0.11.2)