FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2474.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2474.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671952
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35610.21298458328947242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5850.038.40332145
GTCCTAG3250.035.7035521
GTATAAG6250.030.16951
GCCGACC5700.029.242594145
TACAGCG2601.1461998E-725.0950455
CCTAGTG12600.023.5930523
CCCTAGT13950.023.3902632
CCCCTAG13950.022.8745861
CTGCCGC15050.022.150537145
CCGTGTG4058.076313E-1021.4811769
CCTAGAG5700.020.3524133
GTATATA12500.020.3063951
TCCTAGA8900.019.5532072
GTGTATA9450.019.1859341
TAGCACC3802.0816879E-719.0781044
AGTGACC19800.017.940596
TAGTGAC19950.017.8062325
GACCGTG2851.6351427E-417.8051667
TTAGGCT21400.017.6160454
CTAGTGA20400.017.4134464
Produced by FastQC (version 0.11.2)